Cell Line:                              A 549

Cell Description:                 Human lung carcinoma

 

Culture medium:                      Hams F12K or DMEM with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate and 10% FBS

Subculture routine:                 Split confluent cultures 1:3 to 1: 6 i.e. seeding at 2 x 104 to 4 x 104 cells/cm2  using 0,25% trypsin or trypsin / EDTA; 5% CO2, 37¡C

Morphology:                            Epithelial

Karyotype:                              2n = 46

Depositor:                               Obtained from ATCC

Comments:                              Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. They also contain inclusion bodies.

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

 

Biosafety Level:                     1

ECACC number:                      86012804

ATCC designation:                  CCL 185

Reference:                             J Nat Cancer Inst, (1973), 51, 1417; Int J Cancer, (1967), 17, 62