Cell Line:                              A-498

Cell Description:                 Human kidney carcinoma

 

Culture medium:                      Minimum essential medium (Eagle) in Earle's BSS with sodium bicarbonate, 2 mM L-glutamine and 1,0 mM sodium pyruvate, 1% nonessential amino acids, 90%; fetal bovine serum 10%; G6PD, B

Subculture routine:                 Remove medium, rinse with fresh 0.025% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37¡C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Split Ratio:                               A ratio of 1:2 to 1:4 is recommended

Morphology:                            epithelial

Karyotype:                              2n = 46

Comments:                              Derived from a 52 years old female.

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative

Biosafety Level:                     1

Reference:                             Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214,1977. J. Natl. Cancer Inst. 59: 221-226, 1977. Faust JB and Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. Giard DJ et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J.Natl. Cancer Inst. 51: 1417-1423, 1973. Natl. Cancer Inst. Monogr. 49: 5-9, 1978.