Cell Line:                              A 427

Cell Description:                 human lung carcinoma


Culture medium:                      EMEM (EBSS) with sodium bicarbonate, 2 mM L-glutamine, 1,0 mM sodium pyruvate and 1% non essential amino acids (NEAA), 90%; FBS, 10%

Subculture routine:                 Remove medium, rinse fresh 0,25% trypsin solution, remove trypsin and let the culture sit at room temperature (or 37¡C) until the cell detach (about 10 minutes). Add fesh medium, aspirate and dispense into a new flasks. Subculture every 6 or 8 days. A split-ratio of 1:2 to 1:6 is recommended

Fluid Renewal:                        2 times weekly

Morphology:                            Epithelial, monolayer

Karyotype:                              (P60) hypotriploid to hypertriploid with abnormalities including dicentrics, minutes and large subtelocentric marker

Isoenzymes:                           PGM3, 1; PGM, 1-2, ES-D, 1; AK-1,2; GLO-1,1; G6PD, B; Phenotype Frequency Product 0.00006

Donor:                                     male, 52 years, Caucasian

Sterility:                                   Tests for Mycoplasma, bacteria and fungi were negative


Biosafety Level:                     1

References:                           Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214,1977. J. Natl. Cancer Inst. 59: 221-226,1977. Giard DJ et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423,1973.

ATCC number:                        HTB-53